Amido Black 10 B for electrophoresis

ürün Kodu : 101167.0025

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Ürün Grubu : Merck Kimyasalları

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Description
Catalogue Number 101167
Synonyms Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black
Description Amido black 10 B (C.I.20470)
Overview Amido black 10 B (C.I.20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Product Information
CAS number 1064-48-8
EC number 213-903-1
Hill Formula C₂₂H₁₄N₈Na₂O₉S₂
Molar Mass 616.5 g/mol
HS Code 3204 12 00
Structure formula Image Structure formula Image
Quality Level MQ400
Applications
Application for electrophoresis
Physicochemical Information
pH value 9.0 (10 g/l, H₂O, 25 °C)
Bulk density 670 kg/m3
Solubility 10 g/l
Toxicological Information
LD 50 oral LD50 Rat > 5000 mg/kg
Safety Information according to GHS
Storage class 10 - 13 Other liquids and solids
WGK WGK 3 highly hazardous to water
Disposal 3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Storage and Shipping Information
Storage Store at +2°C to +30°C.
Specifications
Dye content (spectrophotometrically) ≥ 80 %
Identity
- sodium
passes test
- UV/VIS-Spectrum passes test
Absorption maximum λ max (water) 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water) 750 - 960
TLC-Test passes test
Loss on drying (110 °C) ≤ 10 %
Suitability for electrophoresis passes test
Suitability as redox indicator passes test

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